Available Holders

Holders Available for the JEOL JEM 3200FS

Although all of the holders described here can also be used in the JEOL JEM 1400plus, some of them are essentially reserved for use with the JEOL JEM 3200FS. Such dual use is noted in the description of the individual holders. However, please do keep in mind that we have established a holder cleaning protocol that must be followed before a holder that has been used in the 1400plus can be used in the 3200FS.

There are resolution test images acquired using the 3200FS associatd with each of the holders described here. It should be noted that the atomic lattice of crystalline gold (2.35 Å) is visible in images recorded using the Gatan CCD camera for all these holders starting at a magnification of 100,000x.

Single Tilt Beryllium Room Temperature Retainer (EM-21150)

This wide tilt retainer is capable of ±15° tilt in the 3200FS. It is intended to be used in situations where the low X-ray signal from the Be surrounding the grid allows users to see elements in a given sample that would be lost in the EDX signal from the other room temperature holders. Though the holder parts visible in the image to the right are made from beryllium, the underside of the plate that locks a grid in place seems to be plated with gold, and there are likely to be gold peaks in some of the EDX spectra recorded using this holder even when the specimen does not contain any gold. This holder is harder to load than the standard room temperature holder and the HTR, but not as difficult as the dual tilt beryllium holder described below. Images showing the resolution that can be attained using this holder in the 3200FS both untilted and at the tilt limits are located on another page:

NOTE: Beryllium (especially any BeO dust) is toxic and extreme care should be taken when handling the single tilt beryllium holder.

JEOL Specimen Quartet Holder (EM-01070 SQH)

This specimen holder is capable of holding up to four grids at the same time and has a mechanism built into the opposite end from where the grids are clamped into position that allows the user to select which grid is in the electron beam. This holder is normally kept with the 1400plus. It is wide like all the previous retainers (except the very narrow high tilt retainer) and can be tilted ±XX° tilt in the 3200FS. Each of the 4 black clamps operates independently and additional images showing how the holder extends and how the clamps open area available on our website through the following link:

Images showing the resolution that can be attained using this holder in the 3200FS both untilted and at the tilt limits will be added to our website soon.

JEOL Dual Tilt Beryllium Holder (EM-31640 STHB)

This dual tilt holder is ideal for situations where the user needs to orient a crystalline specimen along exact zone axis views (for example, electron diffraction or STEM zone axis imaging). This holder is normally kept with the 3200FS. It has a width similar to the standard room temperature holder described above and is thus limited to ±20° tilt around the x-axis in the 3200FS. The 3200FS's pole piece and the tilting mechanism of this holder also allow for ±20° around the y-axis (where + y-tilt means the edge of the grid nearest the holder's tip tilts "down" when the holder is inserted into the column). In addition to these tilting capabilities, the area immediately surrounding the grid is made from beryllium and thus presents a very low background signal for EDX. However, it should be noted here that there may be a small but measurable gold signal in the EDX spectra obtained using this holder: while most of the area surrounding the grid is beryllium, there is a bit of gold also and under some conditions, X-rays from this gold can appear in the EDX spectrum.

NOTE: Beryllium (especially any BeO dust) is toxic and extreme care should be taken when handling the dual tilt beryllium holder.

Fischione Dual Axis Tomography Holder (Model 2040)

This holder is designed for room temperature work with negatively stained specimens or plastic embedded and sectioned material where multiple axis tomography is desired. It is normally kept with the 3200FS. The Fischione 2040 holder operates similarly to a conventional tilt and rotation holder:image of Fischione tomography holder - specimen area the narrow tip of the rod allows for ±60° tilt around the x-axis while the large knob at the opposite end of the holder allows the user to rotate the grid through 360° or more (in the plane of the grid). However, when the small knob on the side of the large knob is tightened into position, the user can rotate the grid by precisely 90° simply by turning the large knob from its current position to the next position (in either direction) where the knob "locks" into place. In addition, the cut-down sides at the tip of the rod provide for a large viewing area even at high tilt. We offer images showing the resolution that can be attained using this holder in the 3200FS both untilted and at its maximum tilt.

Although this holder was designed with biological imaging in mind, there is no reason that it cannot be used for TEM (and STEM) tomography of materials science specimens. The ability to rotate the grid freely while also tilting well beyond the limits of the dual tilt beryllium holder should also make this holder useful for materials science applications when it is desirable to tilt a materials science specimen both beyond the ranges available using the JEOL dual tilt beryllium holder listed above and along specific zone axis views.

ProtoChips Poseidon Select liquid cell holder

Images and description coming soon!

A closeup view of Gatan Model 626 Cryo-Holder showing the open cryo-shutter.
A closeup view of Gatan Model 626 Cryo-Holder showing the closed cryo-shutter.
A closeup edge-on view of Gatan Model 626 Cryo-Holder.
A close-up view of the Gatan Model 914 Cryo-Holder (high tilt for tomography) focusing on the open cryo-shutter.
A close-up view of the Gatan Model 914 Cryo-Holder (high tilt for tomography) focusing on the closed cryo-shutter.
A close-up edge-on view of the Gatan Model 914 Cryo-Holder.