Here is a table of some of the lower magnifications of the JEOL JEM 1400plus and the corresponding pixel sizes for the Gatan OneView camera (as determined using both serialEM and DigitalMicrograph measuring a replica diffraction grating, a so-called waffle grid).
NOTE: serialEM's pixel size calibration routine was used to determine all these pixel sizes. Montaged images were used for the pixel size determination for cases where the magnification was too high for there to be multiple waffle squares in a single CCD frame. Additionally, the following tables feature serialEM pixel sizes which were last calibrated in 2017, and DM pixel size last calibrated on March 22, 2018.
*Pixel sizes shown are calculated (i.e., not directly calibrated) values
The higher magnifications that are calculated instead of calibrated can be calibrated using the lattice spacing of potassium chloroplatinate (0.563 nm), graphite (0.344 nm) or perhaps gold (0.235 nm) on the day of use of the 1400plus.
NOTE: The calibrataed pixel sizes shown in this chart from serialEM and Digital Micrograph almost always differ slightly (or even more than slightly). This is a consequence of differences in the way the pixel size was determined and whether the pixel size is a direct measurement. Calculated pixel sizes are generally less accurate that calibrated ones. In general, the EMC staff trusts the values determined using serialEM more than those of DigitalMicrograph.
