Gatan UltraScan 4000 Current Pixel Sizes

Current (as of July/Aug, 2018)

Here is a table of the 35 lowest magnifications of the JEOL JEM 3200FS and the corresponding pixel sizes for the UltraScan 4000 CCD camera (as determined using serialEM and a replica diffraction grating, a so-called waffle grid).

NOTE: serialEM's pixel size calibration routine was used to determine all the pixel sizes up to 150000x (and the ± error is the standard deviation from multiple measurements either the same day or spread across multiple days). Montaged images were used when the magnification was too high for there to be multiple waffle squares in a single CCD frame (40000x and higher).

NOTE: The pixel sizes changed significantly after mechanical realignment of the 3200FS was done during late summer, 2017. We also offer the calibrated pixel sizes and magnifications from before the realignment in 2017.

Higher magnifications can be calibrated using the lattice spacing of gold or graphite on the day of use of the 3200FS.

NOTE: The pixel size shown in this chart and those reported by Digital Micrograph will almost always differ slightly (or even more than slightly). This is a consequence of differences in the way the pixel size was determined and whether the pixel size is a direct measurement or an interpolated value. In general, the EMC staff trusts the values for magnifications less than 50000x determined using serialEM more than those of DigitalMicrograph. Magnifications starting at 100000x should be calibrated using the gold lattice while magnifications above 50000x should be calibrated using the graphite lattice. Also, keep in mind that when the electron beam is not parallel, the 3200FS shows significant defocus-dependent magnification changes and thus the defocus of specific images will definitely affect the exact magnification of the recorded image.