Here is a table with the nominal magnifications of the JEOL JEM 1010 and the corresponding pixel sizes and calibrated (or calculated) magnifications for the MegaScan 794 CCD camera (determined using DigitalMicrograph and a replica diffraction grating, a so-called waffle grid).
Pixel Sizes for the Gatan MegaScan 794 CCD Camera on the JEOL JEM 1010
Nominal magnification | Pixel size (nm) | Calibrated/calculated magnification |
---|---|---|
50 | 410.488 | 58.47 |
60 | 342.073 | 70.16 |
80 | 256.555 | 93.55 |
100 | 205.244 | 116.93* |
120 | 118.264 | 202.94** |
150 | 137.087 | 175.07* |
200 | 100.865 | 237.94* |
250 | 78.397 | 306.14* |
300 | 61.962 | 387.34* |
400 | 46.974 | 510.93* |
500 | 36.807 | 625.06* |
600 | 30.091 | 797.59* |
800 | 22.629 | 1060.58* |
1000 | 18.560 | 1293.13* |
Nominal magnification | Pixel size (nm) | Calibrated/calculated magnification |
---|---|---|
600 | 24.902 | 963.8* |
750 | 18.907 | 1296.4* |
1000 | 15.037 | 1596.1* |
1200 | 12.781 | 1877.7* |
1500 | 10.467 | 2292.9* |
2000 | 8.459 | 2837.2* |
2500 | 6.680 | 3592.9* |
3000 | 5.576 | 4303.8* |
4000 | 4.352 | 5514.4* |
5000 | 3.506 | 6845.6* |
6000 | 2.921 | 8216.9* |
7500 | 2.363 | 10156.6* |
10000 | 1.733 | 13848.5* |
12000 | 1.453 | 16520.4* |
15000 | 1.172 | 20477.5 |
20000 | 0.879013 | 27303.4 |
25000 | 0.70321 | 34129.2 |
30000 | 0.586008 | 40955.0 |
40000 | 0.439506 | 54606.7 |
50000 | 0.351605 | 68258.4 |
60000 | 0.293004 | 81810.1 |
75000 | 0.234403 | 102387.6 |
100000 | 0.175803 | 136516.8 |
120000 | 0.146502 | 163820.2 |
150000 | 0.117202 | 204775.2 |
200000 | 0.087901 | 273033.6 |
500000 | 0.03516 | 682584.0 |
*These magnifications are determined by DigitalMicrograph during the calibration procedure while the other magnifications are pure calculations
**This magnifications are determined by DigitalMicrograph during the calibration procedure and the recorded calibration image appears to be at a magnification between the 200x and 150x images
Due to the small field of view of the MegaScan 1000 camera, and the camera's large pixel size, it is quit difficult to do calibrations of the camera at magnifications higher than ~12,000x: there are too few (or even less than one) waffle squares to measure their size accurately for mags greater than ~12,000x and only magnifications above 150,000x (where the 1010 has issues with brightness and overall performance) can be used to record the atomic spacings of graphite or crystalline gold. The Electron Microscopy Center (EMC) staff is working on ways to calibrate better the magnifications and pixels sizes for magnifications above 12,000x, and will let users know when things change.
NOTE: The pixel sizes shown in this chart are exactly those reported by Digital Micrograph on June 7, 2016 (and July 26, 2016, for the Low Mag mode data). Further attempts to calibrate the camera are likely to change some of these values.