Some Current Research Using EMC Facilities

Bauer group image Carl Bauer's group uses the cryoTEM facility to visualize photoreceptor proteins  that are involved in bacterial  vision.  The laboratory uses a  combination of biochemistry, X-ray crystallography and now cryo-electron microscopy to decipher interactions that occur between three proteins involved in bacterial vision.
replication fork image from Steve Bell Stephen Bell's group studies various aspects of the molecular biology of Archaea. They are interested in the structural organization of the cell itself and the mechanisms of cell division. In addition, the laboratory is starting to use cryoTEM to elucidate the architecture of the macromolecular complexes that mediate gene transcription and DNA replication.
Bronstein group image Lyudmila Bronstein's group focuses on the formation of inorganic nanoparticles (NPs), their structure, functionalization and applications including catalysis and biorelated studies. Using HRTEM, we will determine the structure of inorganic crystals. Elemental maps of compound NPs (quantum dots, bimetallic NPs, etc.) and their functional shells will be obtained using STEM/EDX capabilities.
Brun group image Bacteria can adopt a myriad of shapes and the mechanisms that generate those changes are only beginning to be understood. Yves Brun's group uses electron cryo tomograpghy (cryoET) to  study the process by which Caulobacter crescentus and related bacteria synthesize a thin extension of the cell envelope called the stalk or  prostheca. This process provides a tractable example of a specific morphological change.
GroEL + GroELS Lingling Chen's group studies protein-protein and protein-DNA interactions in biological processes.  CryoEM is useful in providing  the overall architecture of the macromolecular arrangements.
Kao group image Cheng Kao's group uses the cryoEM facility to analyze the architecture of viruses and perform single particle  reconstruction of protein and protein-RNA complexes.
Kearns group image Bacteria swim by rotating helical, propeller-like, flagella.  Flagellar structure is complex and poorly understood in the Gram positive bacterium Bacillus subtilis. Daniel Kearns' group will genetically dissect components of flagellar assembly and use cryoEM to determine the structural consequences of flagellar mutants.  They are especially interested in determining the structure of the flagella motor.
Mukhopadhyay group image Tuli Mukhopadhyay's group uses cryoEM to determine the structure of different membrane bound RNA viruses (e.g., Sindbis virus).  It is possible to learn how the virus carries out different functions using these structures.
Skrabalak group image Sara Skrabalak's group focuses on the synthesis and application of architecturally and compositionally complex inorganic solids.  The EM Center is instrumental in characterizing the dispersion of different components by elemental mapping using STEM/EDX.
Zlotnick group image Adam Zlotnick's group takes viruses apart and puts them back together. The  self-assembly of virus capsid proteins can be related to virus  biology, development of anti-viral small molecules, and production of  virus-based nanostructures. We will use cryoEM to examine the  molecular details of naturally occurring and synthetic virus capsid  protein structures.

If you would like your research listed here, please contact David Morgan.