Loading Different TEM Holders

Every TEM specimen holder in the EMC needs to be loaded using a different procedure. This series of images shows many of our holders in both the open state (ready to receive a grid) and the closed state (ready to insert into a microscope). The first two holders are prepared for loading by loosening one or more screws, the next two holders have a simple hinged clamping mechanism to lock grids into position and the final holder has a very complicated procedure for loading grids that requires considerable practice to accomplish (especially when the holder is cold).

Closed State

 Open State

 
JEOL Standard Room Temperature Retainer (EM-21020)
image of JEOL standard room temperature holder in closed state image of JEOL standard room temperature holder in open state
 
JEOL High Tilt Retainer (EM-21311)
image of JEOL HTR in closed state image of JEOL HTR in the open state
 
JEOL Quick Release Room Temperature Retainer (EM-11610 QR1)
image of JEOL quick release retainer in closed state image of JEOL quick release retainer in the open state
 
JEOL Specimen Quartet Holder (EM-01070 SQH)
more images are here
image of JEOL specimen quartet holder in closed state image of JEOL specimen quartet holder in the open state
 
Gatan 626 Cryo-Holder
more images are here
image of "closed" Gatan 626 cryo-holder in the cryo-transfer station (grid locked into position) image of "open" Gatan 626 cryo-holder in the cryo-transfer station (ready to load a grid into position)
 
Gatan 914 High Tilt Cryo-Tomography Holder
more images are here
image of "closed" Gatan 914 cryo-holder in the cryo-transfer station (grid locked into position) image of "open" Gatan 914 cryo-holder in the cryo-transfer station (ready to load a grid into position)