Pixel Sizes for the Gatan UltraScan 4000 CCD
Camera on the JEOL JEM 3200FS

Here is a table of the 33 lowest magnifications of the JEOL JEM 3200FS and the corresponding pixel sizes for the UltraScan 4000 CCD camera (as determined using serialEM and a replica diffraction grating, a so-called waffle grid). The values shown in blue correspond to the Low Mag mode of operation while the values in black correspond to the normal Mag mode.

NOTE: It is possible to use serialEM's pixel size calibration routine to determine these pixel sizes. The trick is to use montaged images for magnifications too high for there to be multiple waffle squares in a single CCD frame. That is not how the values shown here were determined, but that is now the best way to do this determination.

Calibrated Pixel Size (TEM Mode)
 
 
magnification
pixel size (nm)
 
 
120
90.65
150
74.90
200
53.29
250
44.22
300
36.94
400
28.50
500
23.08
600
19.41
800
14.65
1000
11.83
1200
9.74
1500
7.77
2000
5.65
2500
4.82
3000
3.94
2500
4.93
3000
3.90
4000
2.65
5000
2.19
6000
1.84
8000
1.45
10000
1.15
12000
0.93
15000
0.74
20000
0.56
25000
0.45
30000
0.38
40000
0.30
50000
0.21
60000
0.18
80000
0.15
100000
0.12
120000
0.11

Higher magnifications can be calibrated using the lattice spacing of gold or graphite on the day of use of the 3200FS.

NOTE: The pixel size shown in this chart and those reported by Digital Micrograph will almost always differ slightly (or even more than slightly). This is a consequence of differences in the way the pixel size was determined and whether the pixel size is a direct measurement or an interpoated value. In general, the EMC staff trusts the values determined using serialEM more than those of DigitalMicrograph. Also, bear in mind that when the electron beam is not parallel, the 3200FS shows significant defocus-dependent magnification changes and thus the defocus of specific images will definitely affect the exact magnification of the recorded image.