Pixel Sizes for the Gatan UltraScan 4000 CCD
Camera on the JEOL JEM 3200FS

Current (Oct 2017)

Here is a table of the 33 lowest magnifications of the JEOL JEM 3200FS and the corresponding pixel sizes for the UltraScan 4000 CCD camera (as determined using serialEM and a replica diffraction grating, a so-called waffle grid). The values shown in blue correspond to the Low Mag mode of operation while the values in black correspond to the normal Mag mode.

NOTE1: serialEM's pixel size calibration routine was used to determine all these pixel sizes. Montaged images were used for the pixel size determination for cases where the magnification was too high for there to be multiple waffle squares in a single CCD frame.

NOTE2: The pixel sizes changed significantly after mechanical realignment of the 3200FS was done during late summer, 2017. Calibrated pixel sizes and magnifications from before that work are located here.

Calibrated Pixel Size (TEM Mode)
 
magnification
pixel size (nm)
 
100
120
150
200
250
300
400
500
600
800
1000
1200
1500
2000
2500
3000
3.94
2500
4.93
3000
4000
5000
6000
8000
10000
12000
15000
20000
25000
30000
40000
50000
60000
80000
100000
120000

Higher magnifications can be calibrated using the lattice spacing of gold or graphite on the day of use of the 3200FS.

NOTE: The pixel size shown in this chart and those reported by Digital Micrograph will almost always differ slightly (or even more than slightly). This is a consequence of differences in the way the pixel size was determined and whether the pixel size is a direct measurement or an interpoated value. In general, the EMC staff trusts the values determined using serialEM more than those of DigitalMicrograph. Also, bear in mind that when the electron beam is not parallel, the 3200FS shows significant defocus-dependent magnification changes and thus the defocus of specific images will definitely affect the exact magnification of the recorded image.