Pixel Sizes for the Gatan UltraScan 4000 CCD
Camera on the JEOL JEM 3200FS

Current (as of July/Aug, 2018)

Here is a table of the 35 lowest magnifications of the JEOL JEM 3200FS and the corresponding pixel sizes for the UltraScan 4000 CCD camera (as determined using serialEM and a replica diffraction grating, a so-called waffle grid). The values shown in blue correspond to the Low Mag mode of operation while the values in black correspond to the normal Mag mode.

NOTE1: serialEM's pixel size calibration routine was used to determine all the pixel sizes up to 150000x (and the ± error is the standard deviation from multiple measurements either the same day or spread across multiple days). Montaged images were used when the magnification was too high for there to be multiple waffle squares in a single CCD frame (40000x and higher).

NOTE2: The pixel sizes changed significantly after mechanical realignment of the 3200FS was done during late summer, 2017. Calibrated pixel sizes and magnifications from before that work are located here.

Calibrated Pixel Size (TEM Mode)
 
magnification
pixel size (nm) ±1 standard deviation
 
100
114.960 (± 0.05%)
120
98.930 (± 0.00%)
150
79.416 (± 0.01%)
200
56.158 (± 0.01%)
250
47.740 (± 0.18%)
300
40.501 (± 0.14%)
400
30.219 (± 0.00%)
500
24.140 (± 0.00%)
600
20.320 (± 0.00%)
800
15.350 (± 0.00%)
1000
12.413 (± 0.04%)
1200
10.130 (± 0.00%)
1500
8.082 (± 0.01%)
2000
5.884 (± 0.01%)
2500
5.003 (± 0.01%)
3000
4.177 (± 0.00%)
2500
4.577 (± 1.92%)
3000
3.652 (± 3.09%)
4000
2.468 (± 1.57%)
5000
2.170 (± 1.64%)
6000
1.897 (± 0.46%)
8000
1.432 (± 1.05%)
10000
1.152 (± 1.11%)
12000
0.952 (± 0.84%)
15000
0.758 (± 0.63%)
20000
0.577 (± 1.39%)
25000
0.467 (± 1.01%)
30000
0.389 (± 1.75%)
40000
0.273 (± 1.35%)
50000
0.183 (± 1.79%)
60000
0.161 (± 2.98%)
80000
0.130 (± 2.44%)
100000
0.105 (± 2.80%)
120000
0.093 (± 1.25%)
150000
0.075 (± 1.77%)
200000
(± %)

Higher magnifications can be calibrated using the lattice spacing of gold or graphite on the day of use of the 3200FS.

NOTE: The pixel size shown in this chart and those reported by Digital Micrograph will almost always differ slightly (or even more than slightly). This is a consequence of differences in the way the pixel size was determined and whether the pixel size is a direct measurement or an interpolated value. In general, the EMC staff trusts the values for magnifications less than 50000x determined using serialEM more than those of DigitalMicrograph. Magnifications starting at 100000x should be calibrated using the gold lattice while magnifications above 50000x should be calibrated using the graphite lattice. Also, keep in mind that when the electron beam is not parallel, the 3200FS shows significant defocus-dependent magnification changes and thus the defocus of specific images will definitely affect the exact magnification of the recorded image.